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[5] Even when fiber intake is adequate, several factors can impair butyrate production: [6] Microbiome disruption from antibiotics, stress, or illness Age-related decline in butyrate-producing bacteria Rapid transit time limiting fermentation Individual variation in bacterial composition Competition from other bacterial metabolic pathways This production variability explains why some people experience dramatic benefits from increasing fiber intake while others see little change despite their best efforts

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Interestingly, MhTyrA strongly preferred NADP + over NAD + cofactor (Figure 3C), like plant TyrAs (Gaines et al., 1982