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The researchers detected CD47 expression in 20.8% of samples, which showed a strong connection with both the age of patients and their metastasis status at diagnosis

Finally, a BCA kit (Beyotime) was adopted to calculate the concentration of protein for normalization of the results at the end of the experiments

By reducing nicotinamide methylation, supports cellular NAD+ availabilitya critical coenzyme for mitochondrial function, energy metabolism, and cellular repair