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The expression and purification of TGP was performed using the same procedure as for ecGFP except that the lysate was heated at 80 C instead of 65 C before affinity chromatography

Whilst the authors acknowledged that other factors such as changes to training in certain populations may have contributed to the improvement, they also draw parallels with the way 5000 m running times began to dramatically fall a few years after erythropoietin (EPO) was introduced to the market