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Patch pipettes (25 M) pulled from borosilicate glass (World Precision Instruments, PG10150-4) were filled with a Cs-based low-Cl internal solution containing (in mM) 135 CsMeSO 3 , 10 HEPES, 1 EGTA, 3.3 QX-314, 4 Mg-ATP, 0.3 Na-GTP, 8 Na 2 -phosphocreatine, 290 mOsm/kg, adjusted to pH 7.3 with CsOH

Our findings demonstrate that this approach is not only feasible but also yields outcomes comparable to those in more resource-rich settings

The result was the averaged data from the first six Ca2+ responses (six trials) of each mouse