For plasma samples, separation was carried out using a non-isocratic method, starting at 23% ACN and climbing to 31 % ACN over 9 minutes, at ambient temperature and a flow rate of 0.3 mL/min
However, once you have the Rx from your healthcare professional, you dont have to travel to his or her office for every injection
Nanoscale 8 , 96149620 (2016)
Pharmacokinetics: Cyanocobalamin is administered intranasally, orally, and parenterally, while hydroxocobalamin is administered only parenterally. Once absorbed, vitamin B12 is highly bound to transcobalamin II, a specific B-globulin carrier protein and is distributed and stored primarily in the liver as coenzyme B12. The bone marrow also stores a significant amount of the absorbed vitamin B12. This vitamin crosses the placenta and is distributed into breast milk. Enterohepatic recirculation conserves systemic stores. The half-life is about 6 days (400 days in the liver). Elimination is primarily through the bile; however, excess cyanocobalamin is excreted unchanged in the urine
