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Interestingly, protease treatment of permeabilized cells destroyed most NS proteins without compromising RdRP activity, suggesting that only a small fraction of NS proteins is actively engaged in RNA replication 80

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[44] demonstrated that acute administration of a MC4R agonist into the medial amygdala in the setting of restraint stress resulted in increased anxiety behavior in rats accompanied by increased c-Fos mRNA expression in medial amygdala MC4R neurons

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