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Tubes containing 26, 8.75, 2.9, 0.97, 0.324 and 0.108 ng of a reference DNA (human genomic DNA from buffy coat, cat # 11691112001 from Sigma-Aldrich) were included in each PCR run so that the quantity of targeted templates in each research sample was determined relative to the reference DNA sample by the standard curve method

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Notably, within the first two hours after infusion, no discernible increases in plasma or urine levels of NAD+ or its metabolites were seen, indicating quick and thorough tissue absorption and/or metabolism (at least for the first 2 hours) (1)

10.1111/febs.13412 40 KempurajD.ThangavelR.NatteruP