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Residual cells, debris were discarded by centrifugation and CM concentrated with concentricon devices (Millipore, Temecula, CA) with a 3-kDa membrane pore cut-off, which eliminates the residual ALCAR
doi: 10.3389/fphar.2026.1752204 Received 22 November 2025 Revised 15 April 2026 Accepted 21 April 2026 Published 15 May 2026 Volume 17 - 2026 Edited by Sathish Kumar Natarajan, University of Nebraska-Lincoln, United States Reviewed by Fabio Vivarelli, University of Bologna, Italy Vijay Radhakrishnan, University of Missouri, United States Updates Copyright 2026 Mahalingam, Kharbanda, Casey and Rasineni
By conjugating therapeutic peptides to these bone-targeting sequences, they can achieve higher local concentrations at the exact site where healing is needed while minimizing systemic exposure
