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Description

CLSM imaging Pre-selected rml1 seedlings were mounted on a slide in a drop of water and immediately transferred to a Zeiss confocal microscope LSM780 (Carl Zeiss Microscopy, www.zeiss.de/mikro)

The tissue fragments were mixed with an equal volume of 1 PBS with collagenase II (Sigma-Aldrich, #C6885, 1 mg/ml), followed by incubation at 37 C for 50 min with shaking