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Subsequently, images were collected with CLSM (LSM-800, ZEISS, Germany) (DAPI, ex = 405 nm, em = 460 nm, Cy5.5, ex = 673 nm, em = 692 nm)

Considering aspartate is mainly synthesized in the mitochondrial matrix through tricarboxylic acid cyle 89 , MOTS-c might be involved in regulating mitochondrial aspartateglutamate metabolism