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Tissue was rinsed with ice-cold phosphate-based saline (PBS) to remove feces and then digested in Dulbeccos Modified Eagle Medium (DMEM) containing 10% FCS, 10 mg/100 ml collagenase A, 2 mg/100 ml Dispase II (both Roche/Sigma-Aldrich, Taufkirchen, Germany) and 7.7 mg/100 ml 1,4-Dithiothreit (Carl-Roth, Karlsruhe, Germany) for 10 min at 37 C

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Moreover, complete loss of H3K27 trimethylation was observed in 67% of spindle and pleomorphic cell subtype and in 95% of Triton subtype malignant peripheral nerve sheath tumors