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A NanoBRET membrane competition binding assay was employed to assess the ability of each peptide to inhibit binding of the fluorescent probe ROX-Ex4 to the GLP-1R N-terminally tagged with nanoluciferase (Nluc)

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In the presence of methylene blue there is a very considerable increase in the production of [14C]carbon dioxide from [14C-1]glucose, and a corresponding decrease in the label in lactate and pyruvate, although the total radioactivity in (lactate + pyruvate + carbon dioxide) is the same as in the control incubation