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This technique involves analyzing the ^13C or ^15N enrichment patterns of metabolites to deduce metabolic fluxes through the pathway

These molecules activate immune-sensing pathways in microglia, including TLR4, P2X7, and cGASSTING, leading to the robust release of pro-inflammatory cytokines such as interleukin-1 (IL-1) and tumor necrosis factor- (TNF-) (Ayton et al., 2013)

They include those for transporting transferrin, LDL (low density lipoprotein), insulin, etc., with transferrin receptor-mediated endocytosis being the most investigated and successful one for developing a brain targeting agent