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This is also supported by previous studies, whereby Gly2Ala in exendin-4 was tolerated, but the converse for GLP-1 (Ala2Gly) reduced affinity 31,32,33

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AC system activation generates an increase of the intracellular secondary messenger, cyclic adenosine monophosphate (cAMP), through the conversion of adenosine triphosphate, which then results in the activation of protein kinase A (PKA) and exchange protein activated by cAMP (Epac) [32,33,34,35]