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Prevention of Erastin-Induced Ferroptosis by GPX4 Activator in HT-1080 Cells HT-1080 cells were cultured in DMEM containing 10% FBS, 1% supplemented non-essential amino acids and 1% Pen-Strep mixture (all from Gibco) and maintained in a humidified atmosphere with 5% CO 2 at 37C

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We clarified in that rule (84 FR 65534) that the payer-specific negotiated charge can be found in other parts of the hospital billing and accounting systems than the chargemaster, or in rate tables or the rate sheets found in hospital in-network contracts with third party payers indicating the agreed upon rates for the provision of various hospital services