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J Biol Chem 251 : 42874293 Pisoni RL, Acker TL, Lisowski KM, Lemons RM, Thoene JG 1990 A cysteine-specific lysosomal transport system provides a major route for the delivery of thiol to human fibroblast lysosomes: possible role in supporting lysosomal proteolysis

The cells were harvested at the early stationary phase which was determined from growth curve based on CO 2 exhaust gas measurements