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Under conditions of acute ischemia and hypoxia, the myocardial genome undergoes extensive remodeling of DNA methylation patterns 75

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In cognitive peptides: substitute when possible in vivo tests by cerebral organoids or primary neuronal cultures ( Replacement ), optimise experimental plan to minimise the number of animals ( Reduction ), use appropriate anaesthesia and analgesia during invasive procedures ( Refinement )

Future research should prioritize large-scale, genotype-stratified trials, explore pathways like NLRP3 inflammasome and TGF-/SMAD signaling, and determine whether combination therapies provide superior efficacy compared to monotherapy