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Gln deprivation can enhance the sensitivity of A2780cis cells to cisplatin by promoting the methylation of the GS promoter, inhibiting GS synthesis, and downregulating intracellular GSH levels ( Furthermore, Gln metabolism can also exert epigenetic influences on tumor resistance

Immediately after, read and record absorbance at 340nm every minute for 5 time points at 25C

Int Biodeterior Biodegrad 65:733743 Ferraz ERA, Umbuzeiro GAG, Caloto-Oliveira A, Chequer FMD, Zanoni MVB, Dorta DJ, Oliveira DP (2011) Differential toxicity of disperse red 1 and disperse red 13 in the Ames test, HepG2 cytotoxicity assay, and Daphnia acute toxicity test

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