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Methods Human cell culture and lysis Human epithelial cervix carcinoma HeLa cells (female) and human retinal pigment epithelial RPE1 cells (female) immortalized with hTERT were purchased from ATCC (CCL-2 and CRL-4002, respectively) and cultured in DMEM (Gibco, Invitrogen) supplemented with 10% fetal bovine serum (Gibco, Invitrogen), 100 U/ml penicillin (Gibco, Invitrogen), 100 g/ml streptomycin (Gibco, Invitrogen) at 37 C in a humidified incubator with 5% CO 2

There is preliminary evidence that non-pharmacological therapies used in mental disorders like ECT are able to induce changes in gut microbiota [241, 242]

These ingredients represent some of the most commercially successful categories in modern skincare