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Relative mRNA levels were calculated by applying the 2 - Ct formula to the normalized results

The data suggested that cells elevated GSH export in response to cystine limitation (Fig

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Of note, in agreement with their mechanisms of action, cell treatments with 354 and VX-497 caused opposite effects in steady state levels of IMPDH2: whereas 354 decreased or totally depleted IMPDH2, VX-497 caused a dose-dependent increase of IMPDH2 levels, by a feedback response of the purine synthesis pathway 49,50,51 (Fig