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Cells were cultured in Dulbeccos Modified Eagles medium (Welgene LM001-05) supplemented with 10% fetal bovine serum (Gibco 16000-044), and 1% penicillin/streptomycin (Gibco 15140-122)

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By defining global and gene-specific DNA 5hmC modifications, we demonstrated that DMF induces 5hmC modifications to influence loci-specific chromatin structure through L2HGDH to reduce the levels of L2HG

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