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Neuroprotection and disease modification by astrocytes and microglia in parkinson disease

N-acetyl cysteine (NAC) is a sulfur-based derivative of the amino acid L-cysteine

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Measurement of hydrogen peroxide production during enzyme reaction of FSP1 The content of H 2 O 2 during in vitro enzyme reaction of FSP1 was determined in aerobic or anaerobic conditions by using a hydrogen peroxide fluorometric assay kit (Elabscience, China), which takes advantage of H 2 O 2 reacting with the fluorescent probe in the presence of peroxidase to generate fluorescence intensity ( ex = 535 / em = 587 nm) that is proportional to the H 2 O 2 concentration range from 0.02 to 10 M