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Efficacy and safety of retatrutide, a GIP, GLP-1, and glucagon receptor agonist, in people with type 2 diabetes and inadequate glycaemic control with diet and exercise (TRANSCEND-T2D-1): a double-blind, randomised, phase 3 trial

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Cells were digested using trypsin and collected in EP tubes, centrifuged, and the supernatant was discarded and resuspended in 500 l 1 PBS containing 2 M C11-BODIPY (581/591) (Thermo, D3861)