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High-pH RP fractionation was performed at a flow rate of 0.6 mL/min with a gradient starting at 5% B for 2 min, increased to 50% B in 12 min, to 70% B within 0.5 min, and held for 6.5 min

Abstract Nuclear factor kappa B (NF-B) plays a central role in the inflammatory process, and activation of Keap1-Nrf2 system counteracts with NF-B by upregulating various genes such as superoxide dismutase (SOD), catalase, and heme oxygenase-1 (HO-1)