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Blood samples in EDTA (for all analyses except glucagon, GLP-1 and glicentin) or P800 tubes (Becton Dickinson, Franklin Lakes, NJ) were immediately placed on ice and centrifuged at 4C and 2500g for 10 minutes, after which plasma was aliquoted and stored at -80C until analysis

Practical travel guidance for transporting retatrutide: Follow written instructions from the trial pharmacy regarding permitted time out of refrigeration and approved transport equipment Use a validated medical-grade cool bag or insulated travel case with a pre-conditioned cool pack pre-condition ice packs according to the manufacturer's instructions to avoid temperatures below 0C Do not place the product directly on ice or ice packs use a separator or cloth to prevent freezing Use a thermometer or temperature data logger inside the cool bag to monitor conditions during transit

Some peptides have become increasingly popular amongst professional and amateur athletes, bodybuilders and gymgoers, without strong evidence of advantages or even safety