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The sections were stained using an Oil Red O staining kit (lot number 20180528, Nanjing Jiancheng Bioengineering), as described previously ( Western Blot Analysis Liver tissue was homogenized in lysis buffer (150 mM NaCl, 1% Nonidet P-40, 0.1% SDS, 50 mM Tris-HCl pH 7.4, 1 mM EDTA, 1 mM PMSF, and 1 Roche complete mini protease inhibitor cocktail)

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The epidemiology of congenital cryptorchidism, testicular ascent and orchiopexy