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Its main function is G-actin sequestration, which means that the peptide binds monomeric actin, controlling the ratio of free actin monomers (G-actin) and polymerized fibers (F-actin)

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It is widely used in early pharmacology research and is endorsed in dose conversion literature (Nair & Jacob, 2016)

These ILK biology studies have contributed to understanding of how TB-500s extracellular signalling , distinct from its intracellular actin sequestration function and contributes to its tissue repair and survival-promoting effects