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Neural pathways: The vagus nerve is the primary neural connection, with afferent nerve fibers sensing mechanical stimuli, chemical signals, and microbial metabolites within the intestinal lumen, transmitting this information to the nucleus tractus solitarius (NTS), which subsequently projects to higher brain regions, including the amygdala, hippocampus, and prefrontal cortex ( Immune pathways: The gut is the largest immune organ in the body, with gut-associated lymphoid tissue (GALT) representing approximately 70% of the entire immune system (Vighi et al., 2008)

The plasma glucose, insulin and glucagon levels were 46.3 21.3 mg/dL, 2439.7 687.9 pg/mL, and 107.3 19.6 pg/mL, respectively