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For plasma samples, separation was carried out using a non-isocratic method, starting at 23% ACN and climbing to 31 % ACN over 9 minutes, at ambient temperature and a flow rate of 0.3 mL/min

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For more on medication timing, How Long Is GLP 1 Good For: Storage, Usage, and Treatment Timelines is a helpful reference

Antidepressants and anti-anxiety medications such as selective serotonin reuptake inhibitors (SSRIs) and serotonin and norepinephrine reuptake inhibitors (SNRIs) can make phentermine less effective, as well as antihistamines and beta blockers, Dr