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Redox Biol (2020) 31:101435

However, when these pathways are also impaired, the UPS and ERAD/UPR are activated as compensatory mechanisms

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coli , we reduced the reported disulfide bond between the active site cysteine C13 and GSH in the clarified cell lysate using dithiothreitol (DTT) and removed the reduced GSH with an extended on-column washing step during purification