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It should be injected about two inches away from the navel itself

Measurement of calcium response Ca 2+ imaging was performed under continuous perfusion of NG neurons with KrebsRinger-Henseleit buffer (Composition in mmol L 1 : NaCl 119, KCl 4.74, CaCl 2 2.54, MgCl 2 1.19, KH 2 PO 4 1.19, NaHCO 3 25, HEPES 10, pH 7.4) at a flow rate of 600 l/min at 37 C