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When AMPK is triggered, it shifts cells toward fat-burning pathways

Puromycin selection was performed to get the stable expression of shRNAs and shNTCs

Moreover, PLGA NPs nanoparticles are formed by PLGA nanoparticles (NPs) that encapsulate heparanase CD4 + and CD8 + T cell epitopes alone or in combination with TLR 3 and 7 ligands as model antigens and then use DEC-205 antibodies to target the ligand to DCs surface molecules can not only target DCs more effectively and be internalized by DCs but also promote T cells proliferation, showing higher tumor killing efficacy in vitro and in vivo ( 209) In summary, nanoparticles composed of polysaccharides or other materials can exert anti-tumor activity by simultaneously regulating DCs and T cells