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However, ITCs such as SFN produced by bacteria during incubation were unstable in the culture medium, so their final concentration was insignificant

L., Mrquez, L., Gonzlez, N., Daz-Miguel, M

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Thereafter, membranes were washed and incubated with horseradish peroxidase-conjugated anti-IgG secondary antibody (Cell Signaling Tech, Frankfurt, DE) followed by detection using a chemiluminescence system (Pierce ECL Western Blotting Substrate, ThermoFisher) and Sapphire Biomolecular Imager (Azure Biosystems)