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in both approaches, cell viability was not affected in the present study

In this study, we combined correlative light and electron microscopy (CLEM), 3D electron tomography (ET), cryo-EM, and all-atom molecular dynamics simulations, along with cell biology and genetic approaches, to investigate plant retrograde transport in the model plant Arabidopsis thaliana , with the following major findings: (1) The CLEM, 3D ET, and immunogold transmission electron microscopy (TEM) analyses revealed distinct spherical vesicles (~3050 nm in diameter) labeled by retromer markers and VSR proteins in Arabidopsis root cells, with nascent vesicles budding from MVBs in Arabidopsis root cells

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