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The reactive thiol of roGFP2 attacks this disulfide and glutathione is transferred to roGFP2 by a disulfide exchange reaction

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Bioconjugate Chem 25:510520 Zimmerman ES, Heibeck TH, Gill A, Li X, Murray CJ, Madlansacay MR, Tran C, Uter NT, Yin G, Rivers PJ et al (2014) Production of site-specific antibody-drug conjugates using optimized non-natural amino acids in a cell-free expression system