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PICOs question The primary research question addressed in this review was: What is the effectiveness of sublingual/oral administration of Vitamin B12 alone or compared to other forms of administration (e.g., intramuscular injection)?

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At indicated times after washout, CMFDA (500 nM) was added for 1 h and the cells were lysed and processed for in-gel fluorescence binding assay, to assess recovery of CMFDA binding to GSTO1

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