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After collagen treatment of the lesion-containing material and sieving, the washed cells were layered over a five-layer discontinuous Nycodenz gradient of 0.5, 1.0, 5.0, 10 and 30% (w/v) Nycodenz and centrifuged at 1200 g for 15 min at 10C [13,14]

Each step of cellular respirationglycolysis in the cytoplasm, the Krebs cycle, and the electron transport chain in the mitochondriarequires the presence of NAD+ and NADH, their redox partner

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