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Statistical analysis Statistical analyses were performed utilizing IBM SPSS Statistics, version 28 (IBM Corp., Armonk, NY, USA)

Chromosome blocks were prepared as previously described (Serrano et al., 1999)

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For instance, the SKSC222 strain, engineered with a synthetic isozyme system, achieved 2.0 g/L of glutathione with a volumetric productivity of 29.85 mg/L/h under fed-batch fermentation conditions (Jeon et al., 2023)