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The strength of the promoter in transgene therapy or modulation of the CRISPRa transcriptional activators will play a key role in determining the appropriate treatment and avoiding unpredictable or undesired treatment consequences

Additionally, GCGR activation may indirectly influence fibrogenesis by modulating the hepatic metabolic milieu, reducing lipotoxicity and oxidative stress that serve as upstream triggers for fibrotic pathways (39, 47)

Following annotation steps, the studied proteins were blasted against the online Kyoto Encyclopedia of Genes and Genomes (KEGG) database ( to retrieve their KEGG orthology identifications, after which they were mapped to pathways in KEGG