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Std 1 Std 2 Std 3 Std 4 Std 5 Std 6 Sample Diluent Volume (l) 475 250 250 250 250 250 Addition Stock Std 1 Std 2 Std 3 Std 4 Std 5 Volume of Addition (l) 25 250 250 250 250 250 GSSG Conc (M) 12.5 6.25 3.125 1.56 0.781 0.391 total GSh Conc (M) 25 12.5 6.25 3.125 1.56 0.781 10 EXPECT ASSAY ARTISTRY ASSAy ProtoCol - enD Point for oxidized Glutathione (GSSG) use the 2VP treated standards, 2VP treated Sample Diluent and 2VP treated samples diluted with Sample Diluent as described previously

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