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The number of Rac1 molecules per EV The number of Rac1 molecules in l-EVs from FBS and those from the serum-free culture medium of HEK293 cells expressing Halo-Rac1 and MIM were quantified, as summarized in Fig

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To determine whether these effects depend on RAMP-containing amylin receptor subtypes (AMY1R and AMY3R), experiments were conducted in HFD-fed WT and RAMP1/3 double knockout (KO) mice