Comp Biochem Physiol B Biochem 105(34):719723
Harvested cells were resuspended in lysis buffer (20 mM HEPES-NaOH pH 7.4, 500 mM NaCl, 2 mM MgCl 2 , 0.5 mM TCEP, 1 mM PMSF), then lysed by sonication
For further information regarding WADAs approach to the routes of administration of glucocorticoids and washout periods, please refer to the Summary of Major Modifications and Explanatory Note
Single-cell RNA-sequencing, metabolomic, proteomic, and transcriptomic analyses, together with immunoprecipitation, mass spectrometry, GST-pulldown assays, oxygen consumption rate assays, and other methods, were used to reveal the mechanism of PRMT1 in renal injury caused by CaOx crystals