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A 50kg individual and a 90kg individual may respond optimally to the same dose because the target is receptor occupancy in the brain, not systemic concentration per kilogram

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Cells were cultured in RPMI-1640 media with 11 mM D-glucose (Gibco) supplemented with 10% fetal bovine serum (FBS), 10 mM HEPES, 1 mM sodium pyruvate, 50 M -mercaptoethanol, and 1% penicillin/streptomycin at 37C in a 95% O 2 / 5% CO 2 incubator

As for every sample volume of erythrocyte fraction was known, it gave us possibility to calculate concentration of NADs and glutathione in initial blood sample before Density gradient separation