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The procedure typically begins with a horizontal incision placed low on the abdomen, just above the pubic area

Other calorie restriction interventions have noted increased alpha diversity (Shannon index) over short-term periods by implementing a high protein diet (30% of daily kcal) ( Differential abundance analysis revealed increases in several genera, including Incertae sedis and Eubacterium fissicatena group , and a decrease in Sellimonas in IF1-P

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The next day, the membranes were washed with TBST 3 times for 5 minutes each and incubated with an anti-mouse secondary antibody (1:5000 dilution) in 5% skim milk for 1 hour at room temperature